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Cloning in Plasmid Vectors: Directional Cloning

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 2020 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,020, page pdb.prot101238 (issn: 1940-3402, eissn: 1559-6095, Copyright policy )
Authors: Michael R, Green; Joseph, Sambrook;

doi: 10.1101/pdb.prot101238

pmid: 33139500

Cloning in Plasmid Vectors: Directional Cloning

Abstract

This protocol describes the standard, old-fashioned but reliable procedure for cloning linear DNA fragments whose ends are incompatible with each other but are compatible with those of the linearized vector.

Keywords

Electrophoresis, Agar Gel, Genetic Vectors, DNA, Recombinant, Escherichia coli, DNA, DNA Restriction Enzymes, Cloning, Molecular, Plasmids

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
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