Immunoprecipitation

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 2017 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,017, page pdb.prot098640 (issn: 1940-3402, eissn: 1559-6095,

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Authors: James, DeCaprio; Thomas O, Kohl;

doi: 10.1101/pdb.prot098640 , 10.1101/pdb.top098509

pmid: 29196601 , 33139503

Immunoprecipitation

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Abstract

This immunoprecipitation protocol details individual steps for the enrichment and purification process of specific proteins from a complex cell lysate using an antibody bound to a solid matrix. Purified antigen(s) can be eluted by various methods, and the resultant protein target can be analyzed and/or identified by numerous assays, including the enzyme-linked immunosorbent assay (ELISA), western blotting, or mass spectrometry.

Keywords

Blotting, Western, Antibody Affinity, Antibodies, Monoclonal, Proteins, Antigen-Antibody Complex, Antibodies, Immunoglobulin G, Animals, Humans, Immunoprecipitation, Electrophoresis, Polyacrylamide Gel, Antigens, Cells, Cultured, Protein Binding

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