Preparation of Antisense Oligonucleotides to Inhibit miRNA Function

descriptionPublicationkeyboard_double_arrow_right Article 01 Feb 2018 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,018, page pdb.prot097527 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Chengjian, Li; Phillip D, Zamore;

doi: 10.1101/pdb.prot097527

pmid: 29438060

Preparation of Antisense Oligonucleotides to Inhibit miRNA Function

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Abstract

This protocol is used to design antisense oligonucleotides (ASOs) for specific inhibition of miRNA function in cultured cells. The incorporation of 2′-O-methyl-modifications in ASOs enhances their potency and resistance to degradation, whereas 3′-terminal cholesterol-conjugation facilitates delivery of ASOs into cells.

Keywords

MicroRNAs, Oligonucleotides, Antisense, Cells, Cultured

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