High-Efficiency Yeast Transformation

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2018 United States English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,018, page pdb.prot094995 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Reece-Hoyes, John S.; Walhout, Albertha J M;

doi: 10.1101/pdb.prot094995

pmid: 29967274

handle: 20.500.14038/49869

High-Efficiency Yeast Transformation

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Abstract

High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete.

Country

United States

Related Organizations

University of Massachusetts Medical School
United States

Keywords

Laboratory and Basic Science Research, Systems Biology, Two-Hybrid System Techniques, 500, Genetics and Genomics, Saccharomyces cerevisiae, DNA, Fungal, 530

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