Illumina Sequencing of Bisulfite-Converted DNA Libraries

descriptionPublicationkeyboard_double_arrow_right Article 16 Nov 2016 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,017, page pdb.prot094870 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Paul M, Lizardi; Qin, Yan; Narendra, Wajapeyee;

doi: 10.1101/pdb.prot094870

pmid: 27852841

Illumina Sequencing of Bisulfite-Converted DNA Libraries

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Abstract

Here we describe a standard MethylC-seq protocol using single-read sequencing on an Illumina Genome Analyzer II platform. The protocol involves ligation of methylated sequencing adaptors to sonicated genomic DNA, gel purification, sodium bisulfite conversion, polymerase chain reaction (PCR) amplification, and sequencing.

Keywords

DNA Ligases, Sulfites, Sequence Analysis, DNA, Polymerase Chain Reaction, DNA Primers

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