Library Quantification Using PicoGreen Fluorometry

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 2016 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,017, page pdb.prot094722 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Elaine, Mardis; W Richard, McCombie;

doi: 10.1101/pdb.prot094722

pmid: 27803277

Library Quantification Using PicoGreen Fluorometry

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Abstract

This protocol describes the quantification of DNA using PicoGreen and a fluorometer to determine the concentration of DNA sample for downstream processing. Because dye-based methods will not detect degraded or short DNA fragments, PicoGreen requires DNAs ≥50 bp, but can less reliably detect fragments as small as 20 bp.

Keywords

Humans, Fluorometry, DNA, Organic Chemicals, Fluorescent Dyes

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Fields of Science (3) View all

medical and health sciences

basic medicine

Fields of Science

medical and health sciences

basic medicine

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