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Solution-Phase Exome Capture

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 2016 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,017, page pdb.prot094680 (issn: 1940-3402, eissn: 1559-6095, Copyright policy )
Authors: Elaine, Mardis; W Richard, McCombie;

doi: 10.1101/pdb.prot094680

pmid: 27803274

Solution-Phase Exome Capture

Abstract

This protocol describes the construction of a paired-end library of genomic DNA (gDNA) and subsequent capture of specific regions of a genome using NimbleGen sequence capture probes and Illumina TruSeq oligos. The captured DNA, purified and quantitated, is appropriate for use as template in Illumina sequencing systems. A procedure is also provided for magnetic AMPure bead cleanup.

Keywords

Exome, DNA, Sequence Analysis, DNA, Molecular Biology, Gene Library

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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