Whole-Genome Sequencing: Manual Library Preparation

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 2016 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,017, page pdb.prot094615 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Elaine, Mardis; W Richard, McCombie;

doi: 10.1101/pdb.prot094615

pmid: 27803280

Whole-Genome Sequencing: Manual Library Preparation

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Abstract

This protocol describes a manual approach for the preparation of genomic DNA libraries suitable for Illumina sequencing. Genomic DNA fragments produced by shearing by sonication are ligated to adaptors and amplified by polymerase chain reaction (PCR). The amplified DNA, separated by size and gel-purified, is suitable for use as template in whole-genome sequencing.

Keywords

Electrophoresis, Sonication, DNA Ligases, DNA, Genomics, Polymerase Chain Reaction, Gene Library

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