Infecting Mouse Embryos by Coculturing with Lentivirus

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2018 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,018, page pdb.prot093922 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Shirley, Pease;

doi: 10.1101/pdb.prot093922

pmid: 29496816

Infecting Mouse Embryos by Coculturing with Lentivirus

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Abstract

Lentiviral vectors make it possible to produce transgenic animals without the need to purchase expensive microinjection equipment because integration of the transgene is possible by coculture of embryos with virus. This protocol describes the infection of fertilized oocytes with lentivirus by coculture.

Keywords

Mice, Microinjections, Lentivirus, Animals, Embryo, Mammalian, Coculture Techniques

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