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ChIP

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 2018 United States Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,018, page pdb.prot082610 (issn: 1940-3402, eissn: 1559-6095,

Authors: Kim, Tae Hoon; Dekker, Job;

doi: 10.1101/pdb.prot082610

pmid: 29610359

handle: 20.500.14038/49860

ChIP

- Summary
- Subjects
- Metrics

Abstract

ChIP is a powerful method for analyzing protein–DNA interactions that occur in vivo. A basic protocol involves immunoprecipitation of the cross-linked nucleoprotein complex and isolating and analyzing the DNA that is associated with the protein of interest. The antibody and chromatin amounts indicated here are for ChIP-chip and ChIP-seq and are ∼10-fold higher than for a standard ChIP assay.

Country

United States

Related Organizations

University of Massachusetts Medical School
United States

Keywords

Chromatin Immunoprecipitation, Laboratory and Basic Science Research, Systems Biology, Humans, DNA, Molecular Biology, Antibodies

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	9
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%