The process of generating new neurons of different phenotype and function from undifferentiated stem and progenitor cells starts at very early stages of development and continues in discrete regions of the mammalian nervous system throughout life. Understanding mechanisms underlying neuronal cell development, biology, function, and interaction with other cells, especially in the neurogenic niche of fully developed adults, is important in defining and developing new therapeutic regimes in regenerative neuroscience. Studying these complex and dynamic processes in vivo is challenging because of the complexity of the nervous system and the presence of many known and unknown confounding variables. However, the challenges could be overcome with simple and robust in vitro models that more or less recapitulate the in vivo events. In this work, we will present an overview of present available in vitro cell-based models of neurogenesis.