AbstractMotivationEmpirical Bayes techniques to genotype polyploid organisms usually either (i) assume technical artifacts are knowna priorior (ii) estimate technical artifacts simultaneously with the prior genotype distribution. Case (i) is unappealing as it places the onus on the researcher to estimate these artifacts, or to ensure that there are no systematic biases in the data. However, as we demonstrate with a few empirical examples, case (ii) makes choosing the class of prior genotype distributions extremely important. Choosing a class that is either too flexible or too restrictive results in poor genotyping performance.ResultsWe propose two classes of prior genotype distributions that are of intermediate levels of flexibility: the class of proportional normal distributions and the class of unimodal distributions. We provide a complete characterization of and optimization details for the class of unimodal distributions. We demonstrate, using both simulated and real data, that using these classes results in superior genotyping performance.Availability and ImplementationGenotyping methods that use these priors are implemented in the updog R package available on the Comprehensive R Archive Network:https://cran.r-project.org/package=updog. All code needed to reproduce the results of this paper is available on GitHub:https://github.com/dcgerard/reproduce_prior_sims.Contactdgerard@american.edu