Chemogenetic Control of Nanobodies
Copyright policy )Chemogenetic Control of Nanobodies
ABSTRACT We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.
- European Molecular Biology Laboratory
- EUROPEAN MOLECULAR BIOLOGY LABORATORY Germany
- École Polytechnique Fédérale de Lausanne EPFL Switzerland
- Paul Scherrer Institute Switzerland
- Universität Wien Austria
Biochemistry & Molecular Biology, Green Fluorescent Proteins, Gene Products, gag, Mitosis, Crystallography, X-Ray, Ligands, Biochemical Research Methods, 106023 Molekularbiologie, Protein Domains, Escherichia coli, Fluorescence Resonance Energy Transfer, Humans, nef Gene Products, Human Immunodeficiency Virus, 106052 Cell biology, Databases, Protein, Immunoglobulin Fragments, validation, DNA, 106023 Molecular biology, Single-Domain Antibodies, Kinetics, HEK293 Cells, Microscopy, Fluorescence, HIV-1, cells, Nanoparticles, 106052 Zellbiologie, fusion proteins, HeLa Cells
Biochemistry & Molecular Biology, Green Fluorescent Proteins, Gene Products, gag, Mitosis, Crystallography, X-Ray, Ligands, Biochemical Research Methods, 106023 Molekularbiologie, Protein Domains, Escherichia coli, Fluorescence Resonance Energy Transfer, Humans, nef Gene Products, Human Immunodeficiency Virus, 106052 Cell biology, Databases, Protein, Immunoglobulin Fragments, validation, DNA, 106023 Molecular biology, Single-Domain Antibodies, Kinetics, HEK293 Cells, Microscopy, Fluorescence, HIV-1, cells, Nanoparticles, 106052 Zellbiologie, fusion proteins, HeLa Cells
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