Chemogenetic Control of Nanobodies
Copyright policy )Chemogenetic Control of Nanobodies
ABSTRACTWe introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.
Green Fluorescent Proteins, Gene Products, gag, Mitosis, DNA, Single-Domain Antibodies, Crystallography, X-Ray, Ligands, Kinetics, HEK293 Cells, Microscopy, Fluorescence, Protein Domains, Escherichia coli, Fluorescence Resonance Energy Transfer, HIV-1, Humans, Nanoparticles, nef Gene Products, Human Immunodeficiency Virus, Databases, Protein, Immunoglobulin Fragments, HeLa Cells
Green Fluorescent Proteins, Gene Products, gag, Mitosis, DNA, Single-Domain Antibodies, Crystallography, X-Ray, Ligands, Kinetics, HEK293 Cells, Microscopy, Fluorescence, Protein Domains, Escherichia coli, Fluorescence Resonance Energy Transfer, HIV-1, Humans, Nanoparticles, nef Gene Products, Human Immunodeficiency Virus, Databases, Protein, Immunoglobulin Fragments, HeLa Cells
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