Interferometric Fluorescent Cross Correlation Spectroscopy
Copyright policy )Interferometric Fluorescent Cross Correlation Spectroscopy
Abstract We present a method utilizing single photon interference and fluorescence correlation spectroscopy (FCS) to simultaneously measure transport of fluorescent molecules within aqueous samples. Our method, within seconds, measures transport in thousands of homogenous voxels (100 nm) 3 and eliminates photo-physical artifacts associated with blinking of fluorescent molecules. A comprehensive theoretical framework is presented and validated by measuring transport of quantum dots, associated with VSV-G receptor along cellular membranes as well as within viscous gels.
- University of Utah United States
- Department of Biology University of Utah United States
- Department of Biology United States
- Department of Physics and Astronomy United States
- UNIVERSITY OF UTAH
Membrane Glycoproteins, Light, Science, Q, Cell Membrane, R, Diffusion, Motion, Interferometry, Spectrometry, Fluorescence, Viral Envelope Proteins, Quantum Dots, Medicine, Humans, Research Article, HeLa Cells
Membrane Glycoproteins, Light, Science, Q, Cell Membrane, R, Diffusion, Motion, Interferometry, Spectrometry, Fluorescence, Viral Envelope Proteins, Quantum Dots, Medicine, Humans, Research Article, HeLa Cells
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