High-Throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
Copyright policy )High-Throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
Abstract Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. Here, we present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.
- Karolinska University Hospital Sweden
- Karolinska Institute Sweden
- University of Regensburg Germany
Chromatin Immunoprecipitation, Methodology Article, ChIPmentation, High-Throughput Nucleotide Sequencing, Cell Count, DNA, QH426-470, Chromatin immunoprecipitation, High-throughput genomics, ChIP-seq, Histone Code, Cell Line, Tumor, Genetics, Humans, Epigenetics, Protein Processing, Post-Translational, TP248.13-248.65, Biotechnology, Gene Library, Protein Binding
Chromatin Immunoprecipitation, Methodology Article, ChIPmentation, High-Throughput Nucleotide Sequencing, Cell Count, DNA, QH426-470, Chromatin immunoprecipitation, High-throughput genomics, ChIP-seq, Histone Code, Cell Line, Tumor, Genetics, Humans, Epigenetics, Protein Processing, Post-Translational, TP248.13-248.65, Biotechnology, Gene Library, Protein Binding
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