Two bacterial strains, designated BSP-53 T and BSP-153 T , were isolated from soil samples collected from Beijing Olympic Forest Park, China. Phylogenomic reconstruction based on core genes robustly positioned the strains as two distinct evolutionary lineages within the genus Acinetobacter . Both strains were Gram-negative, aerobic, non-motile and short rods. Their major cellular fatty acids consisted of summed feature 3 (C 16 : 1  ω 7 c /C 16 : 1  ω 6 c ), C 18 : 1  ω 9 c , C 16 : 0 , C 12 : 0 and C 12 : 0 3-OH. The predominant polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The average nucleotide identity values between the novel strains and their closest Acinetobacter type strains were below 95%, with corresponding digital DNA–DNA hybridization values below 70%. Based on polyphasic taxonomic characterization, including chemotaxonomic, phenotypic, phylogenetic and genomic analysis, we propose that strains BSP-53 T and BSP-153 T represent two novel species of the genus Acinetobacter , for which the names Acinetobacter uliginosus sp. nov. and Acinetobacter halobius sp. nov. are designated. The type strains are BSP-53 T (=CGMCC 1.62070 T =KCTC 8190 T ) and BSP-153 T (=CGMCC 1.62071 T =KCTC 8191 T ), respectively.