Four Gram-stain-positive, rod-shaped and non-spore-forming strains zg-332 T , ZJ293, ZJ405 T and ZJ1691 were isolated from the respiratory tract of Marmota himalayana in the Qinghai–Tibet Plateau of China. Comparative 16S rRNA gene sequence analysis revealed that strain zg-332 T shares 99.6% sequence similarity with strain ZJ293 and is most similar to strain Pauljensenia hongkongensis DSM 15629 T . In contrast, strains ZJ405 T and ZJ1691 share 99.7% similarity with each other and are most closely related to strain Schaalia vaccimaxillae DSM 15804 T and strain Schaalia cardiffensis CCUG 44997 T . Phylogenetic analyses based on 16S rRNA gene sequences confirmed these groupings. The G+C content of strains zg-332 T and ZJ405 T is 35.4 mol% and 57.9 mol%, respectively. Comparative genomic analysis indicated that strains zg-332 T and ZJ293 represent a novel genus in the family Actinomycetaceae , whereas strains ZJ405 T and ZJ1691 represent a novel species within the genus Schaalia . Optimal growth occurred at 35.0 °C, pH 8.0 with 0.5% NaCl (w/v) for strain zg-332 T , and 37.0 °C, pH 8.0 with 0.5–1.5% NaCl (w/v) for strain ZJ405 T . The major fatty acids are C 16 : 0 and C 18 : 1 ω 9 c for both strains zg-332 T and ZJ405 T . The polar lipids for both strains include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unidentified glycolipids, with additional phosphatidyl inositol mannosides, unidentified phospholipid for strain zg-332 T and unidentified lipids for strain ZJ405 T . The respiratory quinone of strain zg-332 T is MK-9(H 2 ) and MK-9(H 4 ), and that of strain ZJ405 T is MK-10(H 4 ). The peptidoglycan of both strains zg-332 T and ZJ405 T contains alanine, glutamic acid, glycine and lysine. Based on the polyphasic analyses, strains zg-332 T (=KACC 22128 T =GDMCC 1.1729 T ) and ZJ293 represent a novel genus and species within the family Actinomycetaceae , for which the name Tongtianella jiaweipingae gen. nov., sp. nov. is proposed; strains ZJ405 T (=GDMCC 1.1953 T =KACC 22129 T ) and ZJ1691 represent a novel species of the genus Schaalia , for which the name Schaalia zhouxuedongae sp. nov. is proposed.