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Halomonas binhaiensis sp. nov., isolated from saline-alkali soil

Authors: Xiaoyan Li; Hegang Lu; Qian Wang; Haixia Yang; Hongpeng Yang; Jiang Wu; Haidong Huang;

Halomonas binhaiensis sp. nov., isolated from saline-alkali soil

Abstract

A Gram-stain-negative, aerobic and rod-shaped bacterium, strain Y2R2T, was isolated from a saline–alkali soil sample collected from Binhai New Area, Tianjin, PR China. Growth of strain Y2R2T was observed at 10–45 °C (optimum, 30 °C), at pH 6.0–11.0 (optimum, pH 9.0) and in the presence of 0–15 % (w/v) NaCl (optimum, 9.0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y2R2T was affiliated with the genus Halomonas and showed the highest similarity to Halomonas huangheensis BJGMM-B45T (99.0%) and Halomonas cupida DSM 4740T (98.4%). The digital DNA–DNA hybridization and average nucleotide identity values of 21.0–22.8 % and 73.3–75.7 % with the closely related species H. huangheensis BJGMM-B45T, H. cupida DSM 4740T, H. ventosae AL12T, H. stenophila N12T and H. litopenaei SYSU ZJ2214T were lower than the threshold recommended for species discrimination. The major respiratory quinone of strain Y2R2T was Q-9 and the major cellular fatty acids consisted of C16 : 0, C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The DNA G+C content of strain Y2R2T was 57.0 mol%. On the basis of this polyphasic taxonomic study, strain Y2R2T is considered to represent a novel species of the genus Halomonas , for which the name Halomonas binhaiensis sp. nov. is proposed. The type strain is Y2R2T (=CGMCC 1.16974T=KCTC 72578T).

Related Organizations
Keywords

DNA, Bacterial, Base Composition, Fatty Acids, Nucleic Acid Hybridization, Sequence Analysis, DNA, Alkalies, Bacterial Typing Techniques, RNA, Ribosomal, 16S, Halomonas, Phylogeny

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Powered by OpenAIRE graph
Found an issue? Give us feedback
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Top 10%
Average
Average
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