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Naturally Occurring Temperature-sensitive Influenza A Viruses of the H1N1 and H3N2 Subtypes

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1980 English Publisher:Microbiology SocietyJournal:Journal of General Virology, volume 48, pages 383-389 (issn: 0022-1317, eissn: 1465-2099,

Authors: G. C. Schild; T. Corcoran; J. S. Oxford;

doi: 10.1099/0022-1317-48-2-383

pmid: 7400776

Naturally Occurring Temperature-sensitive Influenza A Viruses of the H1N1 and H3N2 Subtypes

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Abstract

Seventeen of twenty-six influenza A virus isolates of the H1N1 antigenic subtype and two of eleven H3N2 virus isolates from the 1977-78 season exhibited a ts phenotype, were restricted in plaquing in MDCK cells at 38.5 degrees C compared to 34 degrees C and appeared to be naturally occurring ts mutants. The cut-off temperature for two such ts H1N1 virus isolates was established as 38 degrees C. The ts viruses were as thermostable as non-ts isolates and no complementation was detected between the twelve ts viruses tested. Cloning studies with an H1N1 virus isolate with minimal ts properties indicated the presence of a mixed population of ts+ and ts particles. Analysis of seven recombinants of A/HK/117/77(N1N1) virus indicated that the ts lesion(s) was not located in the NA or HA proteins.

Related Organizations

National Institute for Biological Standards and Control
United Kingdom

Keywords

Recombination, Genetic, Genes, Viral, Influenza A Virus, H3N2 Subtype, Genetic Complementation Test, Temperature, Viral Plaque Assay, Cell Line, Dogs, Influenza A Virus, H1N1 Subtype, Influenza A virus, Mutation, Animals, Antigens, Viral

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	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
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