Infection of Tobacco Mesophyll Protoplasts with Tobacco Mosaic Virus
Copyright policy )Infection of Tobacco Mesophyll Protoplasts with Tobacco Mosaic Virus
Summary Isolated protoplasts from healthy tobacco mesophyll tissue were infected with a Rothamsted culture of the common strain of tobacco mosaic virus. The average yield of virus/infected protoplast was estimated at 1.4 to 5.8 × 106 particles, which was almost as much as found in intact plants. Virus titre was assessed by infectivity assay, electron microscopy, and serological techniques.
- Biotechnology and Biological Sciences Research Council United Kingdom
- Nottingham Trent University United Kingdom
- Rothamsted Research United Kingdom
Ornithine, Nicotiana, Time Factors, Latex, Staining and Labeling, Protoplasts, Stereoisomerism, Virus Replication, Precipitin Tests, Microspheres, Tobacco Mosaic Virus, Microscopy, Electron, Plants, Toxic, Culture Techniques, Plant Diseases
Ornithine, Nicotiana, Time Factors, Latex, Staining and Labeling, Protoplasts, Stereoisomerism, Virus Replication, Precipitin Tests, Microspheres, Tobacco Mosaic Virus, Microscopy, Electron, Plants, Toxic, Culture Techniques, Plant Diseases
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