Introduction Wolff-Parkinson-White (WPW) syndrome is a congenital abnormality that involves an accessory pathway between the atria and the ventricles in addition to the normal atrioventricular node-His pathway. This extra pathway can conduct electrical impulses to the ventricles more quickly and can cause pre-excitation arrhythmias to occur [1]. Three percent to 4% of WPW cases are familial and inherited as an autosomal dominant trait. WPW syndrome has a frequency of 1.5 to 3.1 per 1000 persons in Western countries [2]. The incidence of WPW is higher in men than in women, and it can occur at any age [3]. Therefore, it is inevitable that WPW syndrome can pose a major risk to the small fraction of athletes that have inherited the condition. The following two case reports describe the evaluation of possible WPW syndrome in two Division I collegiate athletes, a female softball player and a female water polo player. Quick detection of the syndrome is vital, and this should be followed by risk stratification and treatment according to the guidelines established by the 36th Bethesda Conference.