<script type="text/javascript">
<!--
document.write('<div id="oa_widget"></div>');
document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=undefined&type=result"></script>');
-->
</script>

COPY SCRIPT

For further information contact us at helpdesk@openaire.eu

Aminoacy1 Transfer RNA Formation. VI. Binding of Cations to Transfer RNA and Its Role in Aminoacy1 Transfer RNA Formation1

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1976Publisher:Oxford University Press (OUP)Journal:The Journal of Biochemistry, volume 80, pages 463-469 (issn: 0021-924X, eissn: 1756-2651,

Authors: Yoshifumi Takeda; Takeo Ohnishi; Yuko Ogiso;

doi: 10.1093/oxfordjournals.jbchem.a131299

pmid: 789364

Aminoacy1 Transfer RNA Formation. VI. Binding of Cations to Transfer RNA and Its Role in Aminoacy1 Transfer RNA Formation1

- Summary
- Subjects
- Metrics

Abstract

The role of cations (polyamines and Mg2+) in isoleucyl-tRNA formation catalyzed by purified isolecuyl-tRNA synthetase [EC 6.1.1.5] from Escherichia coli was studied. It was found that spermine, spermidine, and Mg2+ bind to tRNA and that when bound to these cations, tRNA acts as substrate of aminoacylation without requiring further cations. These findings suggest that the primary function of cations in aminoacyl-tRNA formation is to bind to tRNA to stabilize its structure, not to bind to the enzyme to activate it.

Related Organizations

Osaka University
Japan

Keywords

Isoleucine-tRNA Ligase, Binding Sites, Spermidine, RNA, Transfer, Escherichia coli, Polyamines, Magnesium, Spermine, Transfer RNA Aminoacylation, Isoleucine

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	9
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average