High-throughput polyribosome fractionation
Copyright policy )High-throughput polyribosome fractionation
Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose gradients prepared in deep 96-well plates, the advantage of which is that hundreds of polyribosome fractionations can be performed simultaneously in a tabletop centrifuge.
- Kimmel Cancer Center United States
Reverse Transcriptase Polymerase Chain Reaction, Cell Fractionation, Gene Expression Regulation, Cell Line, Tumor, Polyribosomes, Protein Biosynthesis, Centrifugation, Density Gradient, Humans, Puromycin, RNA, Messenger, Ultracentrifugation
Reverse Transcriptase Polymerase Chain Reaction, Cell Fractionation, Gene Expression Regulation, Cell Line, Tumor, Polyribosomes, Protein Biosynthesis, Centrifugation, Density Gradient, Humans, Puromycin, RNA, Messenger, Ultracentrifugation
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