A subcloning strategy for DNA sequence analysis
Copyright policy )A subcloning strategy for DNA sequence analysis
We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid. Ordered sets of subclones of predetermined overlap are are generated. These can be sequenced directly without further strand- or fragment separation steps.
DNA, Bacterial, Base Sequence, DNA, Viral, DNA, Recombinant, Escherichia coli, Methods, DNA Restriction Enzymes, Cloning, Molecular, Semliki forest virus, Plasmids
DNA, Bacterial, Base Sequence, DNA, Viral, DNA, Recombinant, Escherichia coli, Methods, DNA Restriction Enzymes, Cloning, Molecular, Semliki forest virus, Plasmids
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