Site specific enzymatic cleavage of RNA
Copyright policy )Site specific enzymatic cleavage of RNA
The hybridization of a DNA oligonucleotide a specific tetramer or longer) will direct a cleavage by RNase H (EC 3.1.4.34) to a specific site in RNA. The resulting fragments can then be labeled at their 5' or 3' ends, purified, and sequenced directly. This procedure is demonstrated with two RNA molecules of known sequence: 5.8S rRNA from yeast (158 nucleotides) and satellite tobacco necrosis virus (STNV) RNA (1240 nucleotides).
- Harvard University United States
Oligoribonucleotides, Base Sequence, Nucleic Acid Hybridization, Saccharomyces cerevisiae, Thymus Gland, Endonucleases, Substrate Specificity, Molecular Weight, Ribonucleases, Oligodeoxyribonucleotides, RNA, Ribosomal, Animals, Cattle
Oligoribonucleotides, Base Sequence, Nucleic Acid Hybridization, Saccharomyces cerevisiae, Thymus Gland, Endonucleases, Substrate Specificity, Molecular Weight, Ribonucleases, Oligodeoxyribonucleotides, RNA, Ribosomal, Animals, Cattle
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).417 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 0.1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!