Entrapment of plasmid DNA in liposomes

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1979 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 6, pages 2,697-2,705 (issn: 0305-1048, eissn: 1362-4962,

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Authors: G J, Dimitriadis;

doi: 10.1093/nar/6.8.2697

pmid: 379828

pmc: PMC327886

Entrapment of plasmid DNA in liposomes

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Abstract

The entrapment of plasmid DNA (pMB9) and high molecular weight DNA into large unilamellar liposomes is described. The entrapment of DNA is specific and due to encapsulation of DNA into the aqueous compartment of liposomes. The entrapped Dna, resistant to deoxyribonuclease treatment, could be reisolated from liposomes intact and, as has been shown by transformation assay, it remains biologically active. The advantages of our method and possible applications are discussed.

Related Organizations

National Institute for Medical Research
United Kingdom

Keywords

Molecular Weight, Chemistry, Chemical Phenomena, Liposomes, Escherichia coli, Phosphatidylserines, Plasmids

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