Substrate dependence of the mechanism of EcoRI endonuclease
Copyright policy )Substrate dependence of the mechanism of EcoRI endonuclease
The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double strand cleavage of SV40 DNA occurs without dissociation of the enzyme in only 75% of the catalytic events. This mechanistic difference presumably reflects sequence differences about the EcoRI sites of these DNA's.
- Duke University Hospital United States
- Duke Medical Center United States
- Duke University United States
DNA, Bacterial, Kinetics, Base Sequence, DNA, Viral, Bacteriocin Plasmids, Escherichia coli, DNA Restriction Enzymes, Simian virus 40, Substrate Specificity
DNA, Bacterial, Kinetics, Base Sequence, DNA, Viral, Bacteriocin Plasmids, Escherichia coli, DNA Restriction Enzymes, Simian virus 40, Substrate Specificity
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).72 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!