Overmethylation of DNAs by the EcoRI methylase
Copyright policy )Overmethylation of DNAs by the EcoRI methylase
EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI endonuclease activity, and thus is presumably at or near the EcoRI sequences (5') NAATTN.
- University of Michigan–Ann Arbor United States
- University of Michigan–Flint United States
Site-Specific DNA-Methyltransferase (Adenine-Specific), Base Sequence, Adenine, Osmolar Concentration, DNA Restriction Enzymes, Methyltransferases, Hydrogen-Ion Concentration, Coliphages, Substrate Specificity, Bromodeoxyuridine, DNA, Viral, Polyomavirus
Site-Specific DNA-Methyltransferase (Adenine-Specific), Base Sequence, Adenine, Osmolar Concentration, DNA Restriction Enzymes, Methyltransferases, Hydrogen-Ion Concentration, Coliphages, Substrate Specificity, Bromodeoxyuridine, DNA, Viral, Polyomavirus
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