A new RNA-RNA crosslinking reagent and its application to ribosomal 5S RNA
Copyright policy )A new RNA-RNA crosslinking reagent and its application to ribosomal 5S RNA
The synthesis of a new RNA specific bifunctional crosslinking reagent, 1.4-phenyl-diglyoxal, is described which reacts exclusively with guanosines. The properties of the crosslinked products enabled us to develop a straightforward method for identifying the reacted nucleotides. Results obtained with ribosomal 5S RNA of Escherichia coli demonstrate the formation of an intramolecular crosslink between guanosine-2 and guanosine-112 in the stem region.
- Max Planck Institute for Molecular Genetics Germany
- Max Planck Society Germany
Models, Molecular, Aldehydes, Base Sequence, Chemical Phenomena, Guanosine, Glyoxal, Chemistry, RNA, Ribosomal, Nucleic Acid Conformation, Spectrophotometry, Ultraviolet
Models, Molecular, Aldehydes, Base Sequence, Chemical Phenomena, Guanosine, Glyoxal, Chemistry, RNA, Ribosomal, Nucleic Acid Conformation, Spectrophotometry, Ultraviolet
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