Antibodies specific to a deoxyribodinucleotide sequence

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1977 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 4, pages 2,997-3,006 (issn: 0305-1048, eissn: 1362-4962,

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Authors: S A, Khan; M Z, Humayun; T M, Jacob;

doi: 10.1093/nar/4.9.2997

pmid: 410004

pmc: PMC342629

Antibodies specific to a deoxyribodinucleotide sequence

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Abstract

Antibodies were raised in rabbits against the bovine serum albumin conjugate of dpApT. Analysis by double diffusion in agar gel and quantitative precipitation test showed the presence of antibodies specific to the hapten in the antisera. Quantitative data on the specificity of the antibodies were obtained by studying the inhibition of the binding of 3H-dpApT to the antisera by various nonradioactive mono- and oligonucleotides, using a nitrocellulose membrane binding assay. The antibodies were found to be highly specific for the dinucleotide sequence dpApT. The antibodies were able to bind to synthetic oligonucleotides containing the sequence dpApT and to denatured calf thymus DNA.

Related Organizations

Indian Institute of Science Bangalore
India

Keywords

Immunodiffusion, Oligonucleotides, Serum Albumin, Bovine, Binding, Competitive, Antibodies, Kinetics, Structure-Activity Relationship, Oligodeoxyribonucleotides, Animals, Binding Sites, Antibody, Rabbits

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