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Nucleic Acids Research
Article . 1977 . Peer-reviewed
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Nucleic Acids Research
Article
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Nucleic Acids Research
Article . 1978
Data sources: Europe PubMed Central
https://dx.doi.org/10.1093/nar...
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End labeling of enzymatically decapped mRNA

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1977 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 4, pages 4,165-4,174 (issn: 0305-1048, eissn: 1362-4962, Copyright policy )
Authors: A, Efstratiadis; J N, Vournakis; H, Donis-Keller; G, Chaconas; D K, Dougall; F C, Kafatos;

doi: 10.1093/nar/4.12.4165

pmid: 202926

pmc: PMC343232

End labeling of enzymatically decapped mRNA

Abstract

A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.

Related Organizations
Keywords

Adenosine Triphosphatases, Diphosphates, Kinetics, Isotope Labeling, RNA, Messenger, Plants, Alkaline Phosphatase, Phosphorus Radioisotopes

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 185
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 0.1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 1%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
185
Top 10%
Top 0.1%
Top 1%
gold