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Nucleic Acids Research
Article . 1977 . Peer-reviewed
Data sources: Crossref
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Nucleic Acids Research
Article
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Nucleic Acids Research
Article . 1978
Data sources: Europe PubMed Central
https://dx.doi.org/10.1093/nar...
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Interactions of norharman and harman with DNA

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1977 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 4, pages 3,679-3,686 (issn: 0305-1048, eissn: 1362-4962, Copyright policy )
Authors: K, Hayashi; M, Nagao; T, Sugimura;

doi: 10.1093/nar/4.11.3679

pmid: 593881

pmc: PMC343192

Interactions of norharman and harman with DNA

Abstract

The interactions of norharman (9H-pyrido [3,4-b] indole) and harman (1-methyl-9H-pyrido [3,4-b] indole) with DNA were studied. DNA caused remarkable fluorescence quenching and change in the absorption spectra of the dyes. Scatchard plots obtained by optical titration gave Kd values of 2.2 X 10(-5)M and 7.7 X 10(-6)M, and apparent numbers of binding sites of 0.13/base and 0.12/base for norharman and harman, respectively. Agarose gel electrophoresis of circular DNA, closed in the presence or absence of norharman revealed that the dye intercalates DNA, thereby causing 17 +/- 3 degrees unwinding of the double helix.

Related Organizations
Keywords

Binding Sites, DNA, Superhelical, Spectrum Analysis, DNA, Coliphages, Harmine, Alkaloids, Spectrometry, Fluorescence, DNA, Viral, Nucleic Acid Conformation, DNA, Circular, Carbolines

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    influence
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    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
154
Top 10%
Top 1%
Top 10%
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