Repeating restriction fragments of human DNA
Copyright policy )Repeating restriction fragments of human DNA
Human DNA digested with Hae III showed multiple repeats of a 170 base pair fragment. The most prominent band was the 340 base pair dimer, estimated to be 0.8% of the entire genome. Eco R1 and Hha I yielded fragments with similar electrophoretic mobility to the Hae III dimer. In each case this band was markedly enriched in DNA reassociating at a 0t of less than or equal to 1. Hybridization of the Hae III dimer to gels eluted on to filters demonstrated that the multiple Hae III fragments and Eco R1 fragments contained compatible sequences. These sequences may comprise a distinct subclass of DNA.
- Yale University United States
- YALE UNIVERSITY
- Department of Pathology Yale University School of Medicine United States
- School of Medicine Yale University United States
Adult, Brain Chemistry, Male, Base Sequence, Brain Neoplasms, Placenta, Nucleic Acid Hybridization, DNA, DNA Restriction Enzymes, DNA, Satellite, Embryo, Mammalian, Haemophilus influenzae, Molecular Weight, Organ Specificity, Pregnancy, Escherichia coli, Humans, Female
Adult, Brain Chemistry, Male, Base Sequence, Brain Neoplasms, Placenta, Nucleic Acid Hybridization, DNA, DNA Restriction Enzymes, DNA, Satellite, Embryo, Mammalian, Haemophilus influenzae, Molecular Weight, Organ Specificity, Pregnancy, Escherichia coli, Humans, Female
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