Further characterization of the polynucleotide phosphorylase of Micrococcus luteus
Copyright policy )Further characterization of the polynucleotide phosphorylase of Micrococcus luteus
The purification of polynucleotide phosphorylase from Micrococcus luteus by chromatography on phosphocellulose colums is described. This procedure offers several advantages over previous procedures. Previously determined molecular weights for Form-I enzyme and Form-T enzyme derived from Form-I by limited tryptic hydrolysis were confirmed as 2.7 and 2.3 times 10-5, respectively. Form-I appears homogeneous in the ultracentrifuge, but multiple active protein species are separable by polyacrylamide gel electrophoresis. The multiple species are probably the result of proteolysis. On polyacrylamide gel electrophoresis under denaturing conditions, Form-T yielded a single size of subunit of 71,000 daltons, and Form-I yielded several bands of different molecular sizes. These results differ from earlier determinations. The amino acid compositions of Form-I and Form-T are reported. Form-I contains only between 8 and 10 cysteine residues per molecule and Form-T half that many.
- National Institutes of Health United States
- National Institute of Health Pakistan
Polyribonucleotide Nucleotidyltransferase, Binding Sites, Iodoacetates, Chromatography, Ion Exchange, Micrococcus, Molecular Weight, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Trypsin, Cysteine, Amino Acids, Protein Binding
Polyribonucleotide Nucleotidyltransferase, Binding Sites, Iodoacetates, Chromatography, Ion Exchange, Micrococcus, Molecular Weight, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Trypsin, Cysteine, Amino Acids, Protein Binding
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