Sequences spanning the EcoRI substrate site
Copyright policy )Sequences spanning the EcoRI substrate site
Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the EcoRI site of simian virus 40 DNA and a 15-base-pair sequence overlapping the EcoRI site of Col El plasmid DNA. Three other DNAs were similarly tested, although extended sequences were not determined in these cases. The EcoRI site was shown to be symmetric double-stranded equivalent of -N-G-A-A-T-T-C-N-.
- University of California, Berkeley United States
- University of California San Francisco Medical Center United States
Binding Sites, Deoxyribonucleases, Base Sequence, Phosphoric Diester Hydrolases, Oligonucleotides, DNA, DNA Restriction Enzymes, Endonucleases, Escherichia coli, Methods, Protein Binding
Binding Sites, Deoxyribonucleases, Base Sequence, Phosphoric Diester Hydrolases, Oligonucleotides, DNA, DNA Restriction Enzymes, Endonucleases, Escherichia coli, Methods, Protein Binding
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