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Nucleic Acids Research
Article . 1987 . Peer-reviewed
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Nucleic Acids Research
Article
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Nucleic Acids Research
Article . 1987
Data sources: Europe PubMed Central
https://dx.doi.org/10.1093/nar...
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Electroporation for the efficient transfection of mammalian cells with DNA

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1987 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 15, pages 1,311-1,326 (issn: 0305-1048, eissn: 1362-4962, Copyright policy )
Authors: G, Chu; H, Hayakawa; P, Berg;

doi: 10.1093/nar/15.3.1311

pmid: 3029703

pmc: PMC340526

Electroporation for the efficient transfection of mammalian cells with DNA

Abstract

A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.

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Keywords

Chloramphenicol O-Acetyltransferase, DNA, Bacterial, Genes, Viral, Kanamycin Kinase, Genetic Vectors, Phosphotransferases, Cell Line, Avian Sarcoma Viruses, Genes, Acetyltransferases, Genes, Bacterial, Growth Hormone, Electrochemistry, Humans, Pentosyltransferases, Cloning, Molecular, Genetic Engineering, Plasmids

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    selected citations
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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 856
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 0.1%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
856
Top 1%
Top 0.1%
Top 0.1%
gold