Site-directed cleavage of RNA
Copyright policy )Site-directed cleavage of RNA
Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Escherichia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occurred in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.
- Ajinomoto United States
- Kawasaki Medical School Japan
- Central Research Laboratories (United Kingdom) United Kingdom
- Hokkaido Bunkyo University Japan
Oligoribonucleotides, Base Sequence, Endoribonucleases, Ribonuclease H, Q beta Replicase, Nucleic Acid Conformation, RNA, Hydrogen Bonding, RNA, Double-Stranded
Oligoribonucleotides, Base Sequence, Endoribonucleases, Ribonuclease H, Q beta Replicase, Nucleic Acid Conformation, RNA, Hydrogen Bonding, RNA, Double-Stranded
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