Nucleotide sequence of mouse IL-2 receptor cDNA and its comparison with the human IL-2 receptor sequence
Copyright policy )Nucleotide sequence of mouse IL-2 receptor cDNA and its comparison with the human IL-2 receptor sequence
We have cloned cDNA encoding the mouse interleukin-2 (IL-2) receptor from a murine T cell line, CTLL using human IL-2 receptor cDNA as probe. COS 7 cells transfected with the cDNA expressed the antigen recognized by the monoclonal antibody against the murine IL-2 receptor. The cDNA identified 4 species of mRNA (4.5, 3.5, 2.2 and 1.5 kb) of the mouse IL-2 receptor in CTLL cells. Difference in the length of mRNA seems to be ascribed to the variable length of the 3' untranslated sequence. Total nucleotide sequence (approximately 1400 bp) of this cDNA was determined and compared with the human receptor. The nucleotide and amino acid sequences of the IL-2 receptor are 70% and 60%, respectively, homologous in average between the two species. The comparison has revealed several conserved regions localized to particular exons such as transmembrane and cytoplasmic portions, suggesting that these regions are important for receptor function and its regulation.
- Kyoto University Japan
- Osaka University Japan
- Freie Universität Berlin Germany
Mice, Inbred BALB C, Base Sequence, Receptors, Interleukin-2, DNA, DNA Restriction Enzymes, Cell Line, Mice, Gene Expression Regulation, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Receptors, Immunologic
Mice, Inbred BALB C, Base Sequence, Receptors, Interleukin-2, DNA, DNA Restriction Enzymes, Cell Line, Mice, Gene Expression Regulation, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Receptors, Immunologic
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).124 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!