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Nucleic Acids Research
Article . 1985 . Peer-reviewed
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Nucleic Acids Research
Article
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Nucleic Acids Research
Article . 1985
Data sources: Europe PubMed Central
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https://dx.doi.org/10.1093/nar...
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Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1985 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 13, pages 45-57 (issn: 0305-1048, eissn: 1362-4962, Copyright policy )
Authors: Tomas Kempe; Wesley I. Sundquist; Flora Chow; Shiu-Lok Hu;

doi: 10.1093/nar/13.1.45

pmid: 2582352

pmc: PMC340973

Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides

Abstract

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.

Keywords

Chemical Phenomena, DNA Ligases, Oligonucleotides, Biotin, Collodion, Nucleic Acid Hybridization, RNA Ligase (ATP), DNA, Chemistry, Oligodeoxyribonucleotides, RNA

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    selected citations
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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 111
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
111
Top 10%
Top 1%
Top 1%
gold