Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1983 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Research, volume 11, pages 2,575-2,584 (issn: 0305-1048, eissn: 1362-4962,

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Authors: T, Dörper; E L, Winnacker;

doi: 10.1093/nar/11.9.2575

pmid: 6856470

pmc: PMC325909

Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides

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Abstract

The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides.

Related Organizations

Ludwig-Maximilians-Universität München
Germany

Keywords

Molecular Weight, Chemistry, Chemical Phenomena, Oligodeoxyribonucleotides, Phosphines, Oligonucleotides

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