ABSTRACT We present spatially resolved kinematic measurements of stellar and ionized gas components of dwarf galaxies in the stellar mass range $10^{8.5}\!-\!10^{10} \, \mathrm{M}_{\odot }$, selected from Sloan Digital Sky Survey DR7 and DR8 and followed up with Keck/Low-Resolution Imaging Spectrometer spectroscopy. We study the potential effects of active galactic nuclei (AGNs) on Galaxy-wide gas kinematics by comparing rotation curves of 26 Galaxies containing AGNs, and 19 control Galaxies with no optical or infrared signs of AGNs. We find a strong association between AGN activity and disturbed gas kinematics in the host Galaxies. While star-forming Galaxies in this sample tend to have orderly gas discs that co-rotate with the stars, 73 per cent of the AGNs have disturbed gas. We find that 5 out of 45 Galaxies have gaseous components in counter-rotation with their stars, and all Galaxies exhibiting counter-rotation contain AGNs. Six out of seven isolated Galaxies with disturbed ionized gas host AGNs. At least three AGNs fall clearly below the stellar–halo mass relation, which could be interpreted as evidence for ongoing star formation suppression. Taken together, these results provide new evidence supporting the ability of AGN to influence gas kinematics and suppress star formation in dwarf galaxies. This further demonstrates the importance of including AGN as a feedback mechanism in galaxy formation models in the low-mass regime.