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Microscopy
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Microscopy
Article . 2015 . Peer-reviewed
Data sources: Crossref
Microscopy
Article . 2016
Data sources: Europe PubMed Central
https://dx.doi.org/10.1093/jmi...
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Principles of cryo-EM single-particle image processing

descriptionPublicationkeyboard_double_arrow_right Article 24 Dec 2015 English Publisher:Oxford University Press (OUP)Journal:Microscopy, volume 65, pages 57-67 (issn: 2050-5698, eissn: 2050-5701, Copyright policy )
Authors: Fred J, Sigworth;

doi: 10.1093/jmicro/dfv370

pmid: 26705325

pmc: PMC4749045

Principles of cryo-EM single-particle image processing

Abstract

Single-particle reconstruction is the process by which 3D density maps are obtained from a set of low-dose cryo-EM images of individual macromolecules. This review considers the fundamental principles of this process and the steps in the overall workflow for single-particle image processing. Also considered are the limits that image signal-to-noise ratio places on resolution and the distinguishing of heterogeneous particle populations.

Related Organizations
Keywords

Imaging, Three-Dimensional, Macromolecular Substances, Protein Conformation, Cryoelectron Microscopy, Signal-To-Noise Ratio

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    selected citations
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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 87
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
87
Top 1%
Top 10%
Top 10%
bronze