Human Monoclonal Antibodies to Human Cytomegalovirus

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 1989 English Publisher:Oxford University Press (OUP)Journal:The Journal of Infectious Diseases, volume 159, pages 436-443 (issn: 0022-1899, eissn: 1537-6613,

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Authors: S K, Foung; S, Perkins; P, Bradshaw; J, Rowe; L B, Rabin; G R, Reyes; E T, Lennette;

doi: 10.1093/infdis/159.3.436

pmid: 2536787

Human Monoclonal Antibodies to Human Cytomegalovirus

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Abstract

Human monoclonal antibodies (HMAbs) to human cytomegalovirus (HCMV) have been developed by using electric field-induced cell fusion of human B lymphocytes to the human-mouse cell line SBC-H20. By this procedure, multiple hybridomas have been produced that secrete IgG 1 HMAbs with distinct patterns of indirect immunofluorescence on HCMV-infected cells. HMAbs Z01 and X20 immunoprecipitated a major protein at 64 kDa. HMAb Z02 immunoprecipitated a major protein of 48-50 kDa. HMAb Z10 identified a single protein at 65 kDa and HMAb X16 identified proteins at 100, 65, and 36-38 kDa. The HMAbs demonstrated varying degrees of virus-neutralizing activity. The production of HMAbs to HCMV provides an important approach to studying the human host response to HCMV by elucidating biologically relevant antigens and epitopes. In addition, HMAbs are a potentially unlimited source of relevant human antibodies for treating life-threatening HCMV infection.

Related Organizations

Stanford University
United States

Keywords

Hybridomas, Blotting, Western, Antibodies, Monoclonal, Cytomegalovirus, Fluorescent Antibody Technique, Viral Plaque Assay, In Vitro Techniques, Antibodies, Viral, Precipitin Tests, Cell Fusion, Molecular Weight, Electricity, Neutralization Tests, Humans, Antigens, Viral

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