Studies on both the characterization and purification of the toxic component in crude culture filtrates of Vibrio cholerae were pursued simultaneously since it was felt that knowledge of properties of the toxin would aid in the procedure for purification. Attention was directed primarily towards the factor responsible for the accumulation of fluid in ligated segments of rabbit intestine. This report describes the electrophoretic properties as well as the effect of various enzymes and organic solvents on the toxin. The objective was to develop procedures that could be scaled up easily to handle large quantities of toxin as well as to explore the more elegant chromatographic procedures that are commonly used for laboratoryscale operations to effect a high degree of purification.