Lessons from GNE-deficient embryonic stem cells: sialic acid biosynthesis is involved in proliferation and gene expression
Copyright policy )Lessons from GNE-deficient embryonic stem cells: sialic acid biosynthesis is involved in proliferation and gene expression
Sialic acids are widely expressed as terminal carbohydrates on glycoconjugates of eukaryotic cells. They are involved in a variety of cellular functions, such as cell adhesion or signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE), which catalyzes the first two steps of sialic acid biosynthesis in the cytosol. Inactivation of GNE causes early embryonic lethality. In this study, we analyzed wild-type and GNE-deficient embryonic stem cells from mice. We found for the first time that proliferation is directly correlated with GNE-expression and the cellular sialic acid concentration. Furthermore, we identified growth-related genes that are differentially expressed in GNE-deficient embryonic stem cells compared to wild-type embryonic stem cells.
Genotype, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression, Gene Expression Regulation, Developmental, Hexosamines, Polymerase Chain Reaction, N-Acetylneuraminic Acid, Mice, Phosphotransferases (Alcohol Group Acceptor), Animals, Alleles, Embryonic Stem Cells, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Signal Transduction
Genotype, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression, Gene Expression Regulation, Developmental, Hexosamines, Polymerase Chain Reaction, N-Acetylneuraminic Acid, Mice, Phosphotransferases (Alcohol Group Acceptor), Animals, Alleles, Embryonic Stem Cells, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Signal Transduction
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