Abstract The stimulatory and protective response known as hormesis elicits an often over compensatory response resulting in life-history trait improvements. There are an array of abiotic and biotic agents that have been shown to trigger hormesis; most commonly studied are chemicals, temperature, and low oxygen. Investigations into low-oxygen exposures that activate the hormetic response reveal that insect performance can be dramatically improved by single short low-oxygen events, but the focus of this work has been primarily on short-term, transitory protection afforded by hormesis. Few reports examine whether the effect is longer lasting or lifelong. We previously reported that one hour of anoxia was enough to induce a hormetic response in the alfalfa leafcutting bee, Megachile rotundata (Hymenoptera: Megachilidae). Here, we investigated the long-term effects of this response by looking at starvation resistance, flight, and locomotory activity throughout the life of the adult bees. In addition, we studied the effects of anoxia hormesis on multiple reproductive metrics. Anoxia hormesis had lifelong positive effects for flight in both sexes. We also recorded higher starvation survival in bees that experienced hormesis. This improvement in performance came at a steep reproductive cost (ie reduction in fecundity). However, no costs or benefits were passed to the next generation. We hypothesize that using anoxia hormesis in the context of pollination services by this species should result in bees that are more active in the field, thereby increasing the numbers of visits to flowers throughout their entire life.