Fluorometric Determination of Urinary Metanephrine and Normetanephrine
Copyright policy )Fluorometric Determination of Urinary Metanephrine and Normetanephrine
Abstract A detailed procedure for the quantitative determination of metanephrine and normetanephrine is given. Epinephrine and norepinephrine are eliminated by selective adsorption on an alumina column. Metanephrine and normetanephrine are isolated with a cation-exchange resin. Fluorescence is developed by a two-step ferricyanide oxidation at two different pH values followed by tautomerization. The concentrations of both metanephrine and normetanephrine are quantitated by differential fluorometry.
- Loma Linda University United States
Chromatography, Catecholamines, Research, Fluorometry, Indicators and Reagents, Urine, Oxidation-Reduction, Chemistry Techniques, Analytical, Fluorescence, Metanephrine, Normetanephrine
Chromatography, Catecholamines, Research, Fluorometry, Indicators and Reagents, Urine, Oxidation-Reduction, Chemistry Techniques, Analytical, Fluorescence, Metanephrine, Normetanephrine
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