Abstract Motivation The UK Biobank data holds immense potential for human health research. However, the complex data preparation and interpretation processes often act as barriers for researchers, diverting them from their core research questions. Results We developed LUKB, an R Shiny-based web tool that simplifies UK Biobank data preparation by automating these preprocessing tasks. LUKB reduces preprocessing time and integrates functions for initial data exploration, allowing researchers to dedicate more time to their scientific endeavors. Detailed deployment and usage can be found in the Supplementary Data. Availability and implementation LUKB is freely available at https://github.com/HaiGenBuShang/LUKB.