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Bioscience Biotechnology and Biochemistry
Article . 2021 . Peer-reviewed
License: OUP Standard Publication Reuse
Data sources: Crossref
Bioscience Biotechnology and Biochemistry
Article . 2024
Data sources: Europe PubMed Central
https://dx.doi.org/10.1093/bbb...
Article
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Enzymatic determination of d-alanine with l-alanine dehydrogenase and alanine racemase

descriptionPublicationkeyboard_double_arrow_right Article 24 Aug 2021 English Publisher:Informa UK LimitedJournal:Bioscience, Biotechnology, and Biochemistry, volume 85, pages 2,221-2,223 (eissn: 1347-6947, Copyright policy )
Authors: Hiroyuki, Ashida; Yoshihiro, Sawa; Tohru, Yoshimura;

doi: 10.1093/bbb/zbab148

pmid: 34427628

Enzymatic determination of d-alanine with l-alanine dehydrogenase and alanine racemase

Abstract

ABSTRACT An enzymatic assay system of d-Ala, which is reported to affect the taste, was constructed using alanine racemase and l-alanine dehydrogenase. d-Ala is converted to l-Ala by alanine racemase and then deaminated by l-alanine dehydrogenase with the reduction of NAD+ to NADH, which is determined with water-soluble tetrazolium. Using the assay system, the d-Ala contents of 7 crustaceans were determined.

Related Organizations
Keywords

Alanine, Alanine Dehydrogenase, Alanine Racemase, Animals, NAD, Enzyme Assays

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    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 3
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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Powered by OpenAIRE graph
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
hybrid