Aim: Since the introduction of surgical gloves, one of the main challenges has been to improve donning and wearability. For the wearer, the formation of “glove juice” is problematic. To improve gliding properties for donning the gloves and absorbing sweat, in 1963 Bio-sorb ® cream: sterile powder cream (Johnson & Johnson Medical, Gargrave, Skipton, United Kingdom) was introduced. Individuals subjectively reported a reduction of sweat production inside the glove, however, the actual effect on sweat accumulation and surgical hand antisepsis has not been examined. Methods: Twenty-six volunteers were used in a study designed to examine the effect of a sterile hand cream applied after surgical hand antisepsis on sweat accumulation inside surgical gloves. A woven cotton glove was worn underneath the surgical glove. Weight differences were used to determine the amount of sweat produced. The influence of Bio-sorb on the efficacy of surgical hand antisepsis was tested immediately and after 90 minutes of wear time by the sampling technique as per EN 12791 and by bacterial analysis of glove juice according to tentative final monograph 1994. Results and Discussion: The amount of sweat produced inside the glove was not reduced by the cream (1.07 ± 0.5 g versus control 1.03 ± 0.5 g; p = 0.75). Considering different skin conditions, it may be possible that some wearers might subjectively observe decreased sweat production after using Bio-sorb cream before donning surgical gloves, because sweat production did decrease in 10 of 26 test subjects. Bio-sorb cream did not affect the efficacy of surgical hand antisepsis either immediately or after 90 minutes. Conclusion: Because of possible risks of contamination of the surgical site with cornstarch from Bio-sorb cream in the case of a glove breach and the failed statistical proof of reduced sweat production, the use of Bio-sorb cream should not to be recommended.